Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: MC1568 Inhibits Thimerosal-Induced Apoptotic Cell Death by Preventing HDAC4 Up-Regulation in Neuronal Cells and in Rat Prefrontal Cortex.

doi: 10.1093/toxsci/kfw157

Figure Lengend Snippet: FIG. 2. Effect of HDAC4 on thimerosal-induced cell death in SH-SY5Y cells. A–D, Effect of 24 h of treatment of thimerosal (THIM) at 0.5 mM on HDAC class IIA protein

Article Snippet: For HDAC4 overexpression experiments, cortical neurons were transfected with 15 lg of DNA (HDAC4) (pcDNAHDAC4-FLAG) that was a gift from Tso-Pang Yao (Addgene plasmid # 30485) or the empty vector pcDNA3 (mock) using Lipofectamine in Optimem for 2 h, 24 h before thimerosal treatment (1 mM/24 h in cortical neurons).

Techniques:

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: MC1568 Inhibits Thimerosal-Induced Apoptotic Cell Death by Preventing HDAC4 Up-Regulation in Neuronal Cells and in Rat Prefrontal Cortex.

doi: 10.1093/toxsci/kfw157

Figure Lengend Snippet: FIG. 3. Effect of MC1568 on thimerosal-induced HDAC4 protein increase. A and B, Western blots of HDAC4 protein levels after 24 h of thimerosal (THIM) at 0.5 mM, alone

Article Snippet: For HDAC4 overexpression experiments, cortical neurons were transfected with 15 lg of DNA (HDAC4) (pcDNAHDAC4-FLAG) that was a gift from Tso-Pang Yao (Addgene plasmid # 30485) or the empty vector pcDNA3 (mock) using Lipofectamine in Optimem for 2 h, 24 h before thimerosal treatment (1 mM/24 h in cortical neurons).

Techniques: Western Blot

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: MC1568 Inhibits Thimerosal-Induced Apoptotic Cell Death by Preventing HDAC4 Up-Regulation in Neuronal Cells and in Rat Prefrontal Cortex.

doi: 10.1093/toxsci/kfw157

Figure Lengend Snippet: FIG. 5. Effect of MC1568 on HDAC4 gene and protein expression in cortical neurons and the role of SUMO1 in MC1568-induced HDAC4 decrease. A, qRT-PCR of HDAC4

Article Snippet: For HDAC4 overexpression experiments, cortical neurons were transfected with 15 lg of DNA (HDAC4) (pcDNAHDAC4-FLAG) that was a gift from Tso-Pang Yao (Addgene plasmid # 30485) or the empty vector pcDNA3 (mock) using Lipofectamine in Optimem for 2 h, 24 h before thimerosal treatment (1 mM/24 h in cortical neurons).

Techniques: Expressing, Quantitative RT-PCR

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: MC1568 Inhibits Thimerosal-Induced Apoptotic Cell Death by Preventing HDAC4 Up-Regulation in Neuronal Cells and in Rat Prefrontal Cortex.

doi: 10.1093/toxsci/kfw157

Figure Lengend Snippet: FIG. 6. Effect of MC1568 on the HDAC4, acetyl-histone H4, and cleaved-caspase 3 protein levels in prefrontal cortex (PFC) and on motor behaviors in thimerosal-treated

Article Snippet: For HDAC4 overexpression experiments, cortical neurons were transfected with 15 lg of DNA (HDAC4) (pcDNAHDAC4-FLAG) that was a gift from Tso-Pang Yao (Addgene plasmid # 30485) or the empty vector pcDNA3 (mock) using Lipofectamine in Optimem for 2 h, 24 h before thimerosal treatment (1 mM/24 h in cortical neurons).

Techniques:

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: MC1568 Inhibits Thimerosal-Induced Apoptotic Cell Death by Preventing HDAC4 Up-Regulation in Neuronal Cells and in Rat Prefrontal Cortex.

doi: 10.1093/toxsci/kfw157

Figure Lengend Snippet: FIG. 7. HDAC4 mediated thimerosal-induced neurotoxicity in vitro and neurobehavioral changes in vivo.

Article Snippet: For HDAC4 overexpression experiments, cortical neurons were transfected with 15 lg of DNA (HDAC4) (pcDNAHDAC4-FLAG) that was a gift from Tso-Pang Yao (Addgene plasmid # 30485) or the empty vector pcDNA3 (mock) using Lipofectamine in Optimem for 2 h, 24 h before thimerosal treatment (1 mM/24 h in cortical neurons).

Techniques: In Vitro, In Vivo